Example of pressure flow curve for Capto Core 700 in packed bed. Large targets are excluded from the beads, whereas smaller impurities can enter into the core where they are being captured by the ligands AC 3Ĥ The product is based on a high flow agarose base matrix, which gives good pressure-flow properties (Fig 2). Schematic representation of the principle for Capto Core 700. The octylamine ligand (see Table 1) is both hydrophobic and positively charged in order to interact strongly with most of the impurities over a wide range of ph and salt concentrations. A schematic representation of Capto Core 700 is given in Figure 1. The molecular size cut-off for proteins is approximately M r = which means that targets that are larger than this will pass through the column in the flow-through fraction. This outer layer prevents large targets from binding to the ligands whereas smaller impurities can enter freely into the beads where they are being captured. The core is surrounded by a layer without ligands. Each bead has a core activated with octylamine ligands. 2 Properties of Capto Core 700 Capto Core 700 is based on the core bead concept. BioProcess media cover all purification steps from capture to polishing. Regulatory Support Files (RSF) are available to assist process validation and submissions to regulatory authorities. Secure ordering and delivery routines give a reliable supply of media for production scale. All BioProcess media are produced with validated methods and are tested to meet manufacturing requirements. Due to the core beads concept, Capto Core 700 offers high purity, improved process economy and increased productivity compared to gel filtration in group separation mode by enabling: Up to 100 times higher load Significantly higher flow rates Straightforward optimization and scale upĢ Table of contents 1 BioProcess Media Properties of Capto Core Method optimization Scale-up Column packing Evaluation of column packing Maintenance Ordering information ACģ 1 BioProcess Media BioProcess media are developed and supported for production scale chromatography. The layer prevents the large targets from entering into the beads whereas smaller proteins and impurities enter into the core where they bind to the hydrophobic and positively charged octylamine ligands. Each bead has a ligand-activated core and an outer layer without ligands. The product is based on the core bead concept. 1 GE Healthcare Life Sciences Instructions AC Core beads Capto Core 700 Capto Core 700 chromatography medium is aimed at intermediate purification and polishing of viruses and other large biomolecules in flow-through mode.
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